![]() ![]() The polylinkers or multiple cloning sites are also a part of lacZ (galactosidase) gene. This plasmid also got a cluster of unique restriction sites known as polylinkers or in other words this plasmid has got multiple cloning sites.ĭ. It also has got selectable marker as ampicillin resistance gene.Ĭ. This helps in getting good yield of cloned DNA in short duration.ī. High copy number, as high as nearly hundred copies per bacterial cell. The features of this pUC19 plasmids are as follows:Ī. pUC19 is an example for an E.coli cloning vector. Unique restriction sites, so that the restriction enzymes can be used to cut the plasmid and DNA of interest can be inserted into the plasmid.Ģ. Selectable marker genes, such as antibiotic resistance geneĬ. For an example E.coli plasmid vector, should have following features:ī. Plasmid are used as cloning vectors, include ori site or origin of replication, needed for replication in the bacterial cells. Plasmids are extra chromosomal circular double stranded DNA, present in bacterial cells. Some of the examples for vectors used in recombinant DNA technology are plasmids, phagemids, cosmids, shuttle vectors and much more.ġ. Several types of vectors are being constructed in laboratory each vector has got different molecular properties as well as cloning capacity. In other words vectors are used as vehicle to carry gene of interest. Many types or kinds of cloning vectors are used for the transfer of gene of interest into the desired tissue or cell.
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